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Alternatives in Monoclonal Antibody Production Workshop. The requirement of an adherent cell substratum for the growth of developing plasmacytoma cell in vivo. J Exp Med Methods for reducing the ammonia in hybridoma cell cultures. Factors influencing monoclonal antibody production in mouse ascites fluid.

Hyper-stimulation of monoclonal antibody production by high osmolarity stress in eRDF medium. Oh, M Yap, W. Current protocols in immunology. Purification of monoclonal antibodies from tissue culture medium depleted of IgG. In vivo and in vitro production of monoclonal antibodies: Current possibilities and future perspectives. In vivo and in vitro production of monoclonal antibodies—Introduction. Large-scale production of murine monoclonal antibodies using hollow fiber bioreactors. Points to consider in the manufacture and testing of monoclonal antibody products for human use.

In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermentor. Comparison with in vivo methods.

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Van den Hondel, C. Recent advances in the large-scale production of antibody fragments using lower eukaryotic microorganisms. Res Immunol 6: Alternatives to pristane priming for ascitic fluid and monoclonal antibody production. Comments on Appendix C of the National Institutes of Health response to the petition of the American Anti-Vivisection Society to prohibit the use of animals in the production of monoclonal antibodies.

Cold Spring Harbor Laboratory Press. Monoclonal antibody production in gas-permeable tissue culture bags using serum-free media. Center for Alternatives to Animal Testing: Alternatives in Monoclonal Antibody Production 8: The production of monoclonal antibodies: Are animals still needed? Production of monoclonal antibodies by the ascites method in laboratory animals. Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas. The effect of pristane on ascites tumor formation and monoclonal antibody production. Use of intracellular pH and annexin-V flow cytometric assays to monitor apoptosis and its suppression by bcl-2 over-expression in hybridoma cell culture.

Institutional policies and guidelines on adjuvants and antibody production. Evaluation of hollow fiber bioreactors; as an alternative to murine ascites production for small scale monoclonal antibody production. Monoclonal antibody production in murine ascites: Clinical and pathological features. Lab Anim Sci Laboratory scale production of monoclonal antibodies in a tumbling chamber.

In vitro production of monoclonal antibodies under serum-free conditions using a compact and inexpensive hollow fibre cell culture unit. J Immunol Methods I Cell culture on artificial capillaries. Continuous cultures of fused cells secreting antibody of predefined specificity. Pilot-scale production of murine monoclonal antibodies in agitated, ceramic-matrix, or hollow-fiber cell culture systems. Batch production of monoclonal antibody by large-scale suspension culture.

Glycosylation analysis of a polyreactive human monoclonal IgG antibody derived from a human-mouse heterohybridoma. Mol Immunol 32 8: Linscott's Directory of Immunological Biological Reagents. An alternative to the use of mice for monoclonal antibody production. Antigen binding properties of purified immunoglobulin A and reconstituted secretory immunoglobulin A antibodies. J Biol Chem Effect of culture conditions on IgM antibody structure, pharmacokinetics, and activity.

Monoclonal antibodies - from small to large-scale production.

Membrane-based cell culture technologies: A scientifically and economically satisfactory alternative to malignant ascites production for monoclonal antibodies. A variant of the dextran-binding mouse plasmacytoma J with altered glycosylation of its heavy chain and decreased reactivity with polymeric dextran. Treatment of malignant ascites. Principles and Practice of Oncology.

Monoclonal antibodies: principles and practice.

November 10, ; Washington, D. Refinement of monoclonal antibody production and animal well-being. Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture. J Cell Physiol Comparative biochemical characterization of a human IgM produced in both ascites and in vitro cell culture.

Multiparametric analyses of hybridoma growth on glass cylinders in a packed-bed bioreactor system with internal aeration. Serum-supplemented and serum-free media comparison for mAb production. J Immunol Methods Nov. A review of experimental studies. Colorectal distension as a noxious visceral stimulus: Physiologic and pharmacologic characterization of pseudoaffective reflexes in the rat. Office for Protection from Research Risks. Office for Protection from Research Risks, Nov. Guide for the Care and Use of Laboratory Animals.

Recognition and Alleviation of Pain and Distress. J Immunol Methods Mar. Different culture methods lead to differences in glycosylation of a murine IgG monoclonal antibody. Comparison of in vitro monoclonal antibody production methods with an in vivo ascites production technique.

Principles and Practice of Clinical Research

Contemp Top Lab Anim Sci 37 5: Rapid diuresis in patients with ascites from chronic liver disease: The importance of peripheral edema. Immunochemical studies of polyspecific natural autoantibodies: Charge, lipid reactivity, Fab 2' fragment activity and complement fixation. Enhanced cloning effeciencies of murine hybridomas using human plasma supplemented medium.

Equipment and procedures for production of monoclonal antibodies in culture. PPCR aims to providing a medium for the publication of scientific articles of high quality, thus promoting the work and training of clinical researchers globally. The journal is initially expected to be quarterly and will be open for new submissions from April Check out the rules for submission. As the journal aims to be an international publication, all information and article submission should be formatted in English. We are proud to announce that all new article submission will be processed by an online submission system.

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